MICROBIOLOGY FOR STUDENTS (M4S)

Test Reagents Used in Microbiology Laboratory | M4S M4S Microbiology for Students Print ↑ Top Microbiology • Laboratory Reagents Test Reagents Used in Microbiology Laboratory Preparation and composition of commonly used reagents for routine biochemical and laboratory tests. 6 Reagent Sections Microbiology M4S Notes Expand all Collapse all Clear search 0 of 6 sections opened 0% Contents 1. Catalase 2. Oxidase 3. Indole 4. Methyl Red 5. Voges-Proskauer 6. Bile Salt Quick Reference 1. Catalase Test ▼ Catalase Reagent — 3% H 2 O 2 Component Quantity Hydrogen peroxide 3 mL Distilled water 97 mL Preparation To 97 mL of distilled water, add 3 mL of hydrogen peroxide and mix well...

Preparation of Gram Stain and Normal Saline 1. Gram's Staining (a) Crystal Violet solution Crystal Violet 20.0 g Ammonium Oxalate 9.0 g Ethanol or Methanol 95 ml Distilled Water (D/W) to make 1 litre Preparation: In a clean piece of paper, 20 gm of crystal violet is weighed and transferred to a clean brown bottle. Then, 95 ml of ethanol is added and mixed until the dye is completely dissolved. To the mixture, 9 gm of ammonium oxalate dissolved in 200 ml of D/W is added. Finally the volume is made 1 litre by adding D/W. (b) Lugol’s Iodine Potassium Iodide 20.0 g Iodine 10.0 g Distilled Water 1000 ml Preparation: To 250 ml of D/W, 20 gm of potassium iodide is dissolved. Then 10 gm of iodine is mixed to it until it is dissolved completely. Finally the volume is made 1 litre by adding D/W. (c) Acetone-Alcohol Decolourizer Acetone 500 ml Ethanol (Absolute) 475 ml Distilled Water 25 ml Preparation: To 25 ml D/W, 475 ml of absolute alcohol is adde...

Culture Media Preparation Procedure for Preparation of Culture Media Culture Media Biochemical Media 1. Preparation of Culture Media Nutrient Agar (NA) i. 37 g of the medium is suspended in 1 L distilled water and boiled to dissolve. ii. Autoclave at 121 °C, 15 psi for 15 min. Nutrient Broth (NB) 13 g dissolved in 1 L distilled water. Autoclave at 121 °C for 15 min. MacConkey Agar (MA) i. 55 g in 1 L water; boil to dissolve. ii. Autoclave 121 °C, 15 psi for 15 min. Blood Agar (BA) i. 42.5 g base in 1 L water; autoclave at 121 °C for 15 min. ii. Cool to 40–50 °C, add 50 mL sterile sheep blood, mix, pour plates. Chocolate Agar (CA) i. Pour sterilized BA into plates. ii. Incubate 75 °C for 30 min;...