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Sequencing

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 Determination of nucleotide sequence in DNA is the important technique in molecular biology.  This technique was first developed by the two scientist groups in 1975.  Maxam AM and Gilbert W. American scientists at Havard University developed the nucleotide sequencing technique using the chemical method.  Sanger F, Niklen S and Coulson AR, the Bristish scientists at Cambridge successfully developed this technique using the enzymatic method.  Nowadays these techniques are widely used in molecular biology research.  The strategies for DNA sequencing consists of 4 steps: 1. Preparation of DNA template 2. Sequencing reaction 3. Electrophoresis 4. Analysis 1. Preparation of DNA template The DNA template used for sequencing can be prepared by several methods: a) Gene cloning  It is conventional method.  Isolate and purify genomic DNA.  Cut with appropriate restriction enzyme.  Cut sequencing vector with the same enzyme.  Insert DNA ...
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Procedure for preparation of Culture Media

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Culture Media Preparation | M4S Digital Textbook MICROBIOLOGY FOR STUDENTS (M4S) Culture Media Bacteriology • Laboratory Manual • Digital Learning Chapter Procedure for Preparation of Culture Media A structured laboratory guide covering the preparation of common culture media and biochemical media used in routine microbiological laboratory work. 12 Media Bacteriology Laboratory M4S Expand All Collapse All Print 0 of 12 chapters opened Table of Contents 1. Culture Media 2. Nutrient Agar 3. Nutrient Broth 4. MacConkey Agar 5. Blood Agar 6. Chocolate Agar 7. Mueller-Hinton Agar 8. O/F Medium 9. SIM Medium 10. MR-VP Medium 11. Simmons Citrate ...
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