16 July 2026

Mannitol Salt Agar (MSA)

MSA Interactive Learning App
🧫 Mannitol Salt Agar (MSA) Interactive Learning System

Intended Use

Selective and differential medium for isolation of Staphylococci.

Principle

  • 7.5% NaCl inhibits most bacteria.
  • Mannitol differentiates fermenters.
  • Phenol red turns yellow in acidic conditions.

Composition

ComponentAmount/L
Peptone10 g
Beef Extract1 g
Mannitol10 g
Sodium Chloride75 g
Phenol Red0.025 g
Agar15 g
pH7.4 ±0.2

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