MICROBIOLOGY FOR STUDENTS (M4S)

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How to perform GENE XPERT MTB/RIF Assay?

on June 09, 2018

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GENE XPERT MTB/RIF ASSAY

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Sequencing

 Determination of nucleotide sequence in DNA is the important technique in molecular biology.  This technique was first developed by the two scientist groups in 1975.  Maxam AM and Gilbert W. American scientists at Havard University developed the nucleotide sequencing technique using the chemical method.  Sanger F, Niklen S and Coulson AR, the Bristish scientists at Cambridge successfully developed this technique using the enzymatic method.  Nowadays these techniques are widely used in molecular biology research.  The strategies for DNA sequencing consists of 4 steps: 1. Preparation of DNA template 2. Sequencing reaction 3. Electrophoresis 4. Analysis 1. Preparation of DNA template The DNA template used for sequencing can be prepared by several methods: a) Gene cloning It is conventional method.  Isolate and purify genomic DNA.  Cut with appropriate restriction enzyme.  Cut sequencing vector with the same enzyme.  Insert DNA ...

ANTIBODY

Prepared By: Ms. Hira Sakha Edited and Updated By: Mr. Bikram Malla

Cultural and Biochemical Characteristics of Medically Important Bacteria

Cultural & Biochemical Characteristics of Medically Important Bacteria | M4S M4S Microbiology for Students Digital Textbook Bacteriology • Cultural & Biochemical Identification Cultural and Biochemical Characteristics of Medically Important Bacteria Laboratory culture appearances and biochemical reactions compiled from the supplied MBLOGSTU source material. 10 Bacterial Groups Student Notes Culture Media Biochemical Tests Expand all Collapse all Print 0 of 10 chapters opened Contents 1. Staphylococcus aureus 2. Salmonella Typhi 3. Vibrio cholerae 4. Pseudomonas aeruginosa 5. Escherichia coli 6. Salmonella Paratyphi A 7. Klebsiella pneumoniae 8. Enterobacter cloacae 9. Acinetobacter spp. 10. Proteus mirabilis 🧫 1. Staphylococcus aureus › Colonies on Mannitol Salt Agar (MSA) Picture Credit: Ms. Samjhana Kapali Colonies ...

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